Electron Microscopic Radioautopgraphic Study on Mitochondrial Protein Synthesis in Colonic Epithelial Cells of Aging Mice
ثبت نشده
چکیده
For the purpose of studying the aging changes of macromolecular synthesis in the colonic cells of experimental animals, we studied 10 groups of aging mice during aging from fetal day 19 to postnatal month 24. They were injected with 3H-leucine, a precursor for protein synthesis, sacrificed and the colonic tissues were processed for light and electron microscopic radioautography. On many radioautograms the localization of silver grains demonstrating protein synthesis in colonic epithelial cells in respective aging groups were analyzed qualitatively. The results revealed that the number of mitochondria increased from embryonic day 19 to postnatal adult month 1 and 2, reaching the maximum, then decreased to senile year 1 to 2. On the other hand, the number of labeled mitochondria showing protein synthesis increased from embryonic day 19 to postnatal newborn days, adult month 1 and 2, reaching the maximum and decreased to month 24. To the contrary, the labeling index increased from embryonic day 19 to postnatal day 3, reaching the maximum, then decreased to day 14, increased to month 1, decreased to month 6, increased to month 12 and decreased to month 24, indicating the aging changes. These results demonstrated that intramitochondrial protein synthesis in the colonic epithelial cells increased and decreased again and again for 3 times repeatedly due to aging of individual animals depending upon the cellular activities at respective aging stages
منابع مشابه
Original Article: Electron Microscopic Radioautographic Studies on the RNA Synthesis in the Colonic Epithelial Cells of Aging Mice
For the purpose of studying the aging changes of macromolecular synthesis in the colonic cells of experimental animals, we studied 10 groups of aging mice during aging from fetal day 19 to postnatal month 24. They were injected with 3 H-uridine, a precursor for RNA synthesis, sacrificed and the colonic tissues were taken out, fixed and processed for light and electron microscopic radioautograph...
متن کاملElectron Microscopic Radioautographic Study on Mitochondrial Dna Synthesis in Adrenal Medullary Cells of Aging and Senescent Mice
In order to study the aging changes of intramitochondrial DNA synthesis of mouse adrenal cells, 8 groups of developing ddY mice, each consisting of 3 individuals, total 24, from fetal day 19 to postnatal newborn at day 1, 3, 9, 14, adult at month 1, 2 and 6, were injected with 3H-thymidine, sacrificed 1 hr later and the liver tissues were fixed and processed for electron microscopic radioautogr...
متن کاملElectron microscopic radioautographic study on mitochondrial RNA synthesis in adrenal cells of aging mice
Original Article Mitochondria Mouse Adrenal cortex and medulla EM radioautography RNA synthesis
متن کاملRNA Synthesis in the Tracheal Epithelial Cells of Aging Mice as Revealed by Electron Microscopic Radioautography
متن کامل
Electron microscopic radioautographic study on mitochondrial RNA synthesis in adrenal cortical and medullary cells of aging mice
In order to study the aging changes of intramitochondrial RNA synthesis of mouse adrenal cells, 10 groups of developing and aging mice, each consisting of 3 individuals, total 30, from fetal day 19 to postnatal newborn at day 1, 3, 9, 14, adult at month 1, 2, 6 and senescent animals at month 12 (year 1) and 24 (year 2) were injected with H-uridine, an RNA precursor, sacrificed 1 hr later and th...
متن کامل